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Saccharopolyspora ghardaiensis sp. nov., an extremely halophilic actinomycete isolated from Algerian Saharan soil

Lookup NU author(s): Professor Hans-Peter Klenk

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Abstract

A novel halophilic actinomycete, strain designated H53^T, was isolated from a Saharan soil sample collected from Chaâbet Ntissa, Béni-isguen, Ghardaïa (South of Algeria) and was characterized taxonomically by means of polyphasic approach. Optimal growth was found to occur at 30–35 °C, pH 6–7 and in the presence of 15–25% (w/v) NaCl. The strain was observed to produce abundant aerial mycelium, which formed long chains of rod-shaped spores at maturity, and well developed and fragmented substrate mycelium. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were arabinose and galactose. The predominant menaquinones were found to be MK-9(H₄) and MK-9(H₆). The predominant cellular fatty acids were determined to be iso- and anteiso-C_17:0, iso-C_15:0, and cis9 iso-C_17:1. The diagnostic phospholipid detected was phosphatidylcholine. The morphological and chemotaxonomic characteristics of the strain were consistent with those of members of the genus Saccharopolyspora. Phylogenetic analyses on the basis of the 16S ribosomal RNA (rRNA) gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus Saccharopolyspora. The 16S rRNA sequence similarities between strain H53^T and other members of the genus Saccharopolyspora ranged from 92.1 to 94.3%. The DNA G+C content of strain H53^T was 72.6%. The genotypic and phenotypic data showed that the strain H53^T represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora ghardaiensis sp. nov. is proposed, with the type strain H53^T (=DSM 45606^T=CCUG 63370^T=CECT 8304^T).

Publication metadata

Author(s): Meklat A, Bouras N, Zitouni A, Sabaou N, Mathieu F, Schumann P, Spröer C, Klenk HP

Publication type: Article

Publication status: Published

Journal: Journal of Antibiotics

Year: 2014

Volume: 67

Issue: 4

Pages: 299-303

Print publication date: 01/04/2014

Online publication date: 18/12/2013

ISSN (print): 0021-8820

ISSN (electronic): 1881-1469

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/ja.2013.136

DOI: 10.1038/ja.2013.136

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