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Lookup NU author(s): Dr Ricardo Martins GouveiaORCiD, Professor Che ConnonORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
We describe a bioactive lipopeptide that combines the capacity to promote the adhesion and subsequent self-detachment of live cells, using template-cell-environment feedback interactions. This self-assembling peptide amphiphile comprises a diene-containing hexadecyl lipid chain (C(16)e) linked to a matrix metalloprotease-cleavable sequence, Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln, and contiguous with a cell-attachment and signalling motif, Arg-Gly-Asp-Ser. Biophysical characterisation revealed that the PA self-assembles into 3 nm diameter spherical micelles above a critical aggregation concentration (cac). In addition, when used in solution at 5-150 nM (well below the cac), the PA is capable of forming film coatings that provide a stable surface for human corneal fibroblasts to attach and grow. Furthermore, these coatings were demonstrated to be sensitive to metalloproteases expressed endogenously by the attached cells, and consequently to elicit the controlled detachment of cells without compromising their viability. As such, this material constitutes a novel class of multi-functional coating for both fundamental and clinical applications in tissue engineering.
Author(s): Dehsorkhi A, Gouveia RM, Smith AM, Hamley IW, Castelletto V, Connon CJ, Reza M, Ruokolainen J
Publication type: Article
Publication status: Published
Journal: Soft Matter
Year: 2015
Volume: 11
Issue: 16
Pages: 3115-3124
Print publication date: 28/04/2015
Online publication date: 05/03/2015
Acceptance date: 05/03/2015
Date deposited: 09/06/2015
ISSN (print): 1744-683X
ISSN (electronic): 1744-6848
Publisher: Royal Society of Chemistry
URL: http://dx.doi.org/10.1039/c5sm00459d
DOI: 10.1039/c5sm00459d
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