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Sampling techniques for oviductal and uterine luminal fluid in cattle

Lookup NU author(s): Dr Miguel Velazquez


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Analysis of luminal fluid microenvironments in the reproductive tract is pivotal to elucidate embryo-maternal signaling mechanisms responsible for successful reproduction in mammals, including cattle. Besides facilitating production of an optimized medium for in vitro fertilization and embryo culture in assisted reproductive technologies, screening of luminal fluid constituents in the oviduct and uterus could also provide critical information for elucidation of mechanisms underlying developmental programming. A key issue in this type of research is the sampling of luminal fluids. In this review we discuss the sampling techniques available for bovine species, including a recent in situ technique developed with the Ghent device, which allows rapid recovery of measurable amounts of pure uterine luminal fluid with minimal disturbance to the donor animal.

Publication metadata

Author(s): Velazquez MA, Parrilla I, VanSoom A, Verberckmoes S, Kues W, Niemann H

Publication type: Review

Publication status: Published

Journal: Theriogenology

Year: 2010

Volume: 73

Issue: 6

Pages: 758-767

Print publication date: 01/04/2010

Online publication date: 13/08/2009

Acceptance date: 28/04/2009

ISSN (print): 0093-691X

ISSN (electronic): 1879-3231

Publisher: Elsevier

URL: .

DOI: 10.1016/j.theriogenology.2009.07.004