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Lookup NU author(s): Dr Catherine Mowbray,
Dr Alison Howard,
Emeritus Professor Barry Hirst
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
The process of amplifying DNA by exploiting the polymerase chain reaction (PCR) was first reported in the 1980s by Mullis and colleagues1. This formed the basis for the next step of PCR technology: using dyes to quantify the amount of DNA being produced after each cycle of the reaction. This process became known as quantitative real-time PCR (qPCR) and has been extensively used to assess the expression of genes from a huge variety of species, becoming a fundamental tool in investigative studies within molecular biology. Here, we discuss the use of qPCR to assess the differentiation of hepatic cell models at an early stage to inform model selection for drug development uses.
Author(s): Mowbray C, Howard A, Hirst BH
Publication type: Review
Publication status: Published
Journal: European Pharmaceutical Review
Print publication date: 30/06/2015
Online publication date: 30/06/2015
Acceptance date: 15/06/2015
ISSN (print): 1360-8606
ISSN (electronic): 1759-1279
Publisher: Russell Publishing Ltd