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Quantitative PCR in the assessment of novel hepatic cell models

Lookup NU author(s): Dr Catherine Mowbray, Dr Alison Howard, Emeritus Professor Barry Hirst



This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


The process of amplifying DNA by exploiting the polymerase chain reaction (PCR) was first reported in the 1980s by Mullis and colleagues1. This formed the basis for the next step of PCR technology: using dyes to quantify the amount of DNA being produced after each cycle of the reaction. This process became known as quantitative real-time PCR (qPCR) and has been extensively used to assess the expression of genes from a huge variety of species, becoming a fundamental tool in investigative studies within molecular biology. Here, we discuss the use of qPCR to assess the differentiation of hepatic cell models at an early stage to inform model selection for drug development uses.

Publication metadata

Author(s): Mowbray C, Howard A, Hirst BH

Publication type: Review

Publication status: Published

Journal: European Pharmaceutical Review

Year: 2015

Volume: 20

Issue: 3

Pages: 8-14

Print publication date: 30/06/2015

Online publication date: 30/06/2015

Acceptance date: 15/06/2015

ISSN (print): 1360-8606

ISSN (electronic): 1759-1279

Publisher: Russell Publishing Ltd