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Clonal strain persistence of Candida albicans isolates from Chronic Mucocutaneous Candidiasis patients

Lookup NU author(s): Dr Muhammad Raza, Dr Desa Lilic

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

Chronic mucocutaneous candidiasis (CMC) is a primary immunodeficiency disorder characterised by susceptibility to chronic Candida and fungal dermatophyte infections of the skin, nails and mucous membranes. Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced.We sequenced for multi locus sequence typing (MLST) 42 C. albicans colonies from 6 unrelated CMC patients; multiple colonies were typed from swabs taken from multiple body sites across multiple time points over a 17 month period. Among isolates from each individual patient, our data show clonal and persistent sequence types that were stable over time, identical between multiple infection sites and exhibit azole resistant phenotypes. No shared origin or common source of infection is identified among isolates from these patients. Additionally we performed C. albicans MLST SNP genotype frequency analysis suggestive of a preponderance of previous loss of heterozygosity (LOH) events among persistent and azole resistant colonisers of patients with autoimmune disorders.

Publication metadata

Author(s): Moorhouse AJ, Renisson C, Raza M, Lilic D, Gow NAR

Publication type: Article

Publication status: Published

Journal: PLoS One

Year: 2016

Volume: 11

Issue: 2

Online publication date: 05/02/2016

Acceptance date: 09/12/2015

Date deposited: 11/03/2016

ISSN (electronic): 1932-6203

Publisher: Public Library of Science

URL: http://dx.doi.org/10.1371/journal.pone.0145888

DOI: 10.1371/journal.pone.0145888

PubMed id: 26849050

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