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Lookup NU author(s): Dr Stephen Swioklo, Dr Andrei Constantinescu, Professor Che ConnonORCiD
Despite considerable progress within the cell therapy industry, unmet bioprocessing and logistical challenges associated with the storage and distribution of cells between sites of manufacture and the clinic exist. We examined whether hypothermic (4°C–23°C) preservation of human adipose-derived stem cells could be improved through their encapsulation in 1.2% calcium alginate. Alginate encapsulation improved the recovery of viable cells after 72 hours of storage. Viable cell recovery was highly temperature dependent, with an optimum temperature of 15°C. At this temperature, alginate encapsulation preserved the ability for recovered cells to attach to tissue culture plastic on rewarming, further increasing its effect on total cell recovery. On attachment, the cells were phenotypically normal, displayed normal growth kinetics, and maintained their capacity for trilineage differentiation. The number of cells encapsulated (up to 2 Å~106 cells per milliliter) did not affect viable cell recovery nor did storage of encapsulated cells in a xenofree, serum-free, current Good Manufacturing Practice-grade medium. We present a simple, low-cost system capable of enhancing the preservation of human adipose-derived stem cells stored at hypothermic temperatures, while maintaining their normal function. The storage of cells in this manner has great potential for extending the time windows for quality assurance and efficacy testing, distribution between the sites of manufacture and the clinic, and reducing the wastage associated with the limited shelf life of cells stored in their liquid state.
Author(s): Swioklo S, Constantinescu A, Connon CJ
Publication type: Article
Publication status: Published
Journal: STEM CELLS Translational Medicine
Year: 2016
Volume: 5
Issue: 3
Pages: 339-349
Print publication date: 01/03/2016
Online publication date: 29/01/2016
Acceptance date: 16/12/2015
Date deposited: 02/02/2016
ISSN (print): 2157-6564
ISSN (electronic): 2157-6580
Publisher: AlphaMed Press, Inc.
URL: http://dx.doi.org/10.5966/sctm.2015-0131
DOI: 10.5966/sctm.2015-0131
PubMed id: 26826163
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