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Lookup NU author(s): Dr William ReidORCiD,
Dr Andrew Close,
Professor Stephen Rushton
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. The immune response against pathogen challenge depends on the specific threat to the host. Here we show that broiler chickens mount a breed dependent immune response to Campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and Bayesian structural equation models (SEM). SEM provides a framework by which cytokine interdependencies, based on prior knowledge, can be tested. In both breeds important cytokines including pro-inflammatory interleukin (IL)-1β, IL-6, IL-4, IL-17A, interferon (IFN)-γ and anti-inflammatory IL-10 and transforming growth factor (TGF)-β4 were are expressed post-challenge. The SEM revealed a putative regulatory pathway illustrating a T helper (Th)17 response and regulation of IL-10, which is breed dependent. The prominence of the Th17 pathway indicates the cytokine response aims to limit the invasion or colonisation of an extracellular bacterial pathogen but the time-dependent nature of the response differs between breeds.
Author(s): Reid WDK, Close AJ, Humphrey S, Chaloner G, Lacharme-Lora L, Rothwell L, Kaiser P, Williams NJ, Humphrey TJ, Wigley P, Rushton SP
Publication type: Article
Publication status: Published
Journal: Royal Society Open Science
Online publication date: 16/03/2016
Acceptance date: 16/02/2016
Date deposited: 18/03/2016
ISSN (electronic): 2054-5703
Publisher: The Royal Society Publishing
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