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Actinomadura algeriensis sp nov., an actinobacterium isolated from Saharan soil

Lookup NU author(s): Professor Hans-Peter Klenk

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Abstract

During the course of a screening programme for new taxa of actinobacteria, a strain designated ACD1(T), was isolated from a Saharan soil in the Hoggar region (Algeria). The taxonomic position of this strain was determined using a polyphasic taxonomic approach. The strain was observed to form extensively branched, non-fragmenting substrate mycelium, and aerial mycelium with straight to flexuous, hooked and irregular spirals (1-2 turns) forming short chains of spores. The diamino acid present in the cell wall is meso-diaminopimelic acid. Galactose, glucose, madurose, mannose and ribose occur in whole-cell hydrolysates. The diagnostic phospholipids detected were diphosphatidylglycerol and phosphatidylinositol. The major menaquinones were identified as MK-9 (H-4) and MK-9 (H-2). The major fatty acids were found to be C-16:0, C-18:1 cis9, iso-C-16:0 and 10-methyl C-18:0. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Actinomadura, and is closely related to Actinomadura sediminis DSM 45500(T) (98.5 % similarity) and Actinomadura cremea subsp. cremea DSM 43676(T) (98.3 % similarity). However, DNA-DNA hybridization revealed only 48.0 % relatedness with A. sediminis DSM 45500(T) and 33.2 % relatedness with A. cremea subsp. cremea DSM 43676(T). The combined phenotypic and genotypic data showed that the strain represents a novel species of the genus Actinomadura, for which the name Actinomadura algeriensis sp. nov. is proposed, with the type strain ACD1(T) (= DSM 46744(T) = CECT 8841(T)).


Publication metadata

Author(s): Lahoum A, Bouras N, Mathieu F, Schumann P, Sproer C, Klenk HP, Sabaou N

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2016

Volume: 109

Issue: 1

Pages: 159-165

Print publication date: 01/01/2016

Online publication date: 05/11/2015

Acceptance date: 31/10/2015

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer

URL: http://dx.doi.org/10.1007/s10482-015-0617-x

DOI: 10.1007/s10482-015-0617-x


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