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Bounagaea algeriensis gen. nov., sp nov., an extremely halophilic actinobacterium isolated from a Saharan soil of Algeria

Lookup NU author(s): Professor Hans-Peter Klenk

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Abstract

A novel halophilic actinobacterium strain, designated H8(T), was isolated from a Saharan soil sample collected in El-Gol,a, South Algeria. Strain H8(T) was identified as representing a new genus using a polyphasic taxonomic approach. Phylogenetic analysis revealed that strain H8(T) shared the highest degree of 16S rRNA gene sequence similarity with 'Mzabimyces algeriensis' DSM 46680(T) (93.0 %), Saccharopolyspora ghardaiensis DSM 45606(T) (91.2 %), Halopolyspora alba DSM 45976(T) (90.8 %) and Actinopolyspora mortivallis DSM 44261(T) (90.0 %). The strain was found to grow optimally at 28-35 A degrees C, at pH 6.0-7.0, and in the presence of 15-25 % (w/v) NaCl. The substrate mycelium was observed to be well developed and fragmented in liquid medium and on solid medium. The aerial mycelium was observed to be moderately abundant and to form long chains with non-motile, smooth-surfaced and ovoid or spherical spores at maturity. The cell wall of strain H8(T) was found to contain meso-diaminopimelic acid. The whole-cell hydrolysates were found to mainly contain arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholine, and MK-9(H-4), MK-9(H-2) and MK-10(H-2) were found to be the predominant menaquinones. The major cellular fatty acids were determined to be anteiso-C-17:0 and iso-C-15:0. The genomic DNA G+C content of strain H8(T) was determined to be 71.3 mol%. The genotypic and phenotypic data showed that the strain represents a novel genus and species, for which the name Bounagaea algeriensis gen. nov., sp. nov. is proposed, with the type strain H8(T) (=DSM 45966(T) = CECT 8470(T)).


Publication metadata

Author(s): Meklat A, Bouras N, Mokrane S, Zitouni A, Schumann P, Sproer C, Klenk HP, Sabaou N

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2015

Volume: 108

Issue: 2

Pages: 473-482

Print publication date: 01/08/2015

Online publication date: 07/06/2015

Acceptance date: 02/06/2015

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer

URL: http://dx.doi.org/10.1007/s10482-015-0500-9

DOI: 10.1007/s10482-015-0500-9


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