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Levels of synthesis of primate-specific nuclear proteins differ between growth-arrested and proliferating cells

Lookup NU author(s): Dr Soren Nielsen

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Abstract

A monoclonal antibody that reacts specifically with the proliferation-sensitive nuclear proteins, isoelectric focusing (IEF) 8Z30 and 8Z31 (molecular weight (MW), 76000 charge variants, HeLa protein catalogue number) has been characterized. As determined by indirect immunofluorescence, the antibody stains the nucleolus and nucleoplasm of interphase-cultured cells of primate origin, but does not react with cells of other species. Proteins having similar MWs and isoelectric points as the human or monkey (primates) proteins were not observed in cultured cells of the following species: aves, bat, dog, dolphin, goat, hamster, mink, mouse, pisces, potoroo, rabbit and rat. Quantitative two-dimensional (2D) gel electrophoretic analysis of [35S]methionine-labelled proteins synthesized by normal (quiescent, proliferating) and SV40-transformed human MRC-5 fibroblasts revealed significant differences in the levels of synthesis of both IEF 8Z30 and 8Z31. In quiescent cells the main labelled product corresponded to IEF 8Z31 (ratio IEF 8Z31/8Z30, 2.3), while in the transformed cells the major product was IEF 8Z30 (ratio, 0.62). Normal proliferating fibroblasts exhibited similar levels of both proteins (ratio, 1.21). Combined levels of synthesis of both proteins were 1.50 and 1.20 times as high in the transformed cells as in the quiescent and proliferating cellls, respectively. Similar results were observed in other pairs of normal and transformed human cells, such as WI38/WI38 SV40 and amnion/AMA. Modulation of the levels of synthesis of these proteins may play a role in cell proliferation.


Publication metadata

Author(s): Celis JE, Madsen P, Nielsen S, Ratz GP, Lauridsen JB, Celis A

Publication type: Article

Publication status: Published

Journal: Experimental Cell Research

Year: 1987

Volume: 168

Issue: 2

Pages: 389-401

Print publication date: 01/02/1987

Online publication date: 22/10/2004

ISSN (print): 0014-4827

ISSN (electronic): 1090-2422

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/0014-4827(87)90011-5

DOI: 10.1016/0014-4827(87)90011-5


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