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Multiple fatty acid binding to albumin in human blood plasma

Lookup NU author(s): Dr Soren Nielsen

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Abstract

Binding equilibria of long-chain fatty acids to human serum albumin, in serum or plasma, were studied by a dialysis exchange rate technique. Palmitate was added to citrated plasma in vitro and it was observed that between six and ten palmitate molecules were bound to albumin with nearly equal affinity. Observation in vivo gave similar results in the following series: (a) in two volunteers with increased fatty acid concentrations after fasting, exercise, and a cold shower: (b) in three male volunteers in whom high concentrations of non-esterified fatty acids, up to 4.6 mM, were induced by intravenous administration of a preparation of lecithin/glycocholate mixed micelles, and (c) in 81 patients with diabetes mellitus, type I.The binding pattern of palmitate in serum or plasma is essentially different from that observed with palmitate added to buffered solutions of pure albumin when two molecules are tightly bound and about four additional molecules with lower affinity. The differences may partly be explained by the presence of chloride ions in blood plasma, reducing the affinity for binding of the first two fatty acid molecules, and partly by facilitated binding of several molecules of mixed fatty acids, as found in plasma.


Publication metadata

Author(s): Brodersen R, Andersen S, Vorum H, Nielsen S, Pedersen AO

Publication type: Article

Publication status: Published

Journal: European Journal of Biochemistry

Year: 1990

Volume: 189

Issue: 2

Pages: 343-349

Print publication date: 01/04/1990

Online publication date: 03/03/2005

ISSN (print): 1742-464X

ISSN (electronic): 1742-4658

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1111/j.1432-1033.1990.tb15495.x

DOI: 10.1111/j.1432-1033.1990.tb15495.x


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