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Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-Phase in cultured cells - some applications of PCNA antibodies

Lookup NU author(s): Dr Soren Nielsen

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Abstract

Immunofluorescence studies using PCNA autoantibodies specific for the proliferation-sensitive protein cyclin have revealed dramatic changes in the nuclear distribution of this protein during the S-phase of normal and transformed cells. Patterns of cyclin antigen distribution subdivide S-phase and have provided new cell cycle landmarks. Some of these (nucleolar exclusion or staining), mimic topographical patterns of DNA synthesis thus arguing for a role of this protein in some specific aspect of DNA replication.Cells outside S-phase (G0 included) stain only weakly with PCNA antibodies, stressing the usefulness of this reagent for identifying proliferating cells (S-phase cells) of both normal and malignant origins.


Publication metadata

Author(s): Celis JE, Madsen P, Nielsen S, Celis A

Publication type: Article

Publication status: Published

Journal: Leukemia Research

Year: 1986

Volume: 10

Issue: 3

Pages: 237-249

Print publication date: 01/02/1986

Online publication date: 22/04/2004

ISSN (print): 0145-2126

ISSN (electronic): 1873-5835

Publisher: Pergamon Press

URL: http://dx.doi.org/10.1016/0145-2126(86)90021-4

DOI: 10.1016/0145-2126(86)90021-4


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