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An Analytical Pipeline for Quantitative Characterization of Dietary Intake: Application To Assess Grape Intake

Lookup NU author(s): Professor John Mathers


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Lack of accurate dietary assessment in free-living populations requires discovery of new biomarkers reflecting food intake qualitatively and quantitatively to objectively evaluate effects of diet on health. We provide a proof-of-principle for an analytical pipeline to identify quantitative dietary biomarkers. Tartaric acid was identified by nuclear magnetic resonance spectroscopy as a dose-responsive urinary biomarker of grape intake and subsequently quantified in volunteers following a series of 4-day dietary interventions incorporating 0 g/day, SO g/day, 100 g/day, and 150 g/day of grapes in standardized diets from a randomized controlled clinical trial. Most accurate quantitative predictions of grape intake were obtained in 24 h urine samples which have the strongest linear relationship between grape intake and tartaric acid excretion (r(2) = 0.90). This new methodological pipeline for estimating nutritional intake based on coupling dietary intake information and quantified nutritional biomarkers was developed and validated in a controlled dietary intervention study, showing that this approach can improve the accuracy Of estimating nutritional intakes.

Publication metadata

Author(s): Garcia-Perez I, Posma JM, Chambers ES, Nicholson JK, Mathers JC, Beckmann M, Draper J, Holmes E, Frost G

Publication type: Article

Publication status: Published

Journal: Journal of Agricultural and Food Chemistry

Year: 2016

Volume: 64

Issue: 11

Pages: 2423-2431

Print publication date: 23/03/2016

Online publication date: 24/02/2016

Acceptance date: 24/02/2016

ISSN (print): 0021-8561

ISSN (electronic): 1520-5118

Publisher: American Chemical Society


DOI: 10.1021/acs.jafc.5b05878


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Funder referenceFunder name
Wellcome Trust NIHR Clinical Research Facility
Integrative Mammalian Biology (IMB) Capacity Building Award
National Institute for Health Research (NIHR)