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Lookup NU author(s): Dr Moritz von Stosch
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Background Non-negative linear combinations of elementary flux modes (EMs) describe all feasible reaction flux distributions for a given metabolic network under the quasi steady state assumption. However, only a small subset of EMs contribute to the physiological state of a given cell. Results In this paper, a method is proposed that identifies the subset of EMs that best explain the physiological state captured in reaction flux data, referred to as principal EMs (PEMs), given a pre-specified universe of EM candidates. The method avoids the evaluation of all possible combinations of EMs by using a branch and bound approach which is computationally very efficient. The performance of the method is assessed using simulated and experimental data of Pichia pastoris and experimental fluxome data of Saccharomyces cerevisiae. The proposed method is benchmarked against principal component analysis (PCA), commonly used to study the structure of metabolic flux data sets. Conclusions The overall results show that the proposed method is computationally very effective in identifying the subset of PEMs within a large set of EM candidates (cases with ~100 and ~1000 EMs were studied). In contrast to the principal components in PCA, the identified PEMs have a biological meaning enabling identification of the key active pathways in a cell as well as the conditions under which the pathways are activated. This method clearly outperforms PCA in the interpretability of flux data providing additional insights into the underlying regulatory mechanisms.
Author(s): von Stosch M, Rodrigues de Azevedo C, Luis M, Feyo de Azevedo S, Oliveira R
Publication type: Article
Publication status: Published
Journal: BMC Bioinformatics
Year: 2016
Volume: 17
Online publication date: 04/05/2016
Acceptance date: 26/04/2016
Date deposited: 05/05/2016
ISSN (electronic): 1471-2105
Publisher: BioMed Central Ltd.
URL: http://dx.doi.org/10.1186/s12859-016-1063-0
DOI: 10.1186/s12859-016-1063-0
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