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In Situ Transesterification of Wet Marine and Fresh Water Microalgae for Biodiesel Production and Its Effect on the Algal Residue

Lookup NU author(s): Kamoru Salam, Dr Sharon Velasquez OrtaORCiD, Professor Adam Harvey



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


This article reports a high yielding technique of synthesizing zirconium dodecyl sulphate (“ZDS”)for in situ transesterification of Nannochloropsis occulata and Chlorella vulgaris for fatty acid methylester (FAME) production. ZDS produced a significantly higher FAME yield in N. occulata thanin C. vulgaris (p = 0.008). The varying performance of ZDS in the two species could be due to theirdifferent cell wall chemistries. Sodium dodecyl sulphate (SDS) in H2SO4 for FAME enhancementfrom the two species was also studied. Treatment with SDS in H2SO4 increased the FAME productionrate in both species. Residual protein content after the in situ transesterification in C. vulgarisand N. occulata reduced respectively by 6.5% and 10%. The carbohydrate content was reduced by71% in C. vulgaris and 65% in N. occulata. The water tolerance of the process when using H2SO4,with or without SDS, was evaluated by hydrating the two species with 10% - 30% distilled water(w/w dry algae). The FAME concentration began to diminish only at 30% water content in bothspecies. Furthermore, the presence of a small amount of water in the biomass or methanol increasedthe lipid extraction efficiency, improving the FAME yield, rather than inhibiting the reaction.

Publication metadata

Author(s): Salam KA, Velasquez-Orta SB, Harvey AP

Publication type: Article

Publication status: Published

Journal: Journal of Sustainable Bioenergy Systems

Year: 2016

Volume: 6

Issue: 2

Pages: 17-30

Print publication date: 01/06/2016

Online publication date: 06/05/2016

Acceptance date: 03/05/2016

Date deposited: 07/06/2016

ISSN (print): 2165-400X

ISSN (electronic): 2165-4018

Publisher: Scientific Research Publishing


DOI: 10.4236/jsbs.2016.62003


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