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Assessing corneal biomechanics with Brillouin spectro-microscopy

Lookup NU author(s): Dr Ricardo Martins GouveiaORCiD, Dr Ana Gouveia Gil, Professor Che ConnonORCiD

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).

Abstract

A new Brillouin spectro-microscope was designed and built to investigate the mechanical properties of bovine and human corneas. This instrument integrates a single-stage virtually imaged phased array spectrometer with a novel adaptive-optics interferometric filter to achieve unprecedented rejection of the elastic background signal. As a result, highly-resolved, reproducible data from both thin and thick collagen-based materials were obtained. In particular, this technique is capable of rigorously measuring the relative stiffness of different areas of human corneas, thus providing a true non-contact method to characterise the fundamental mechanical features of both live and fixed biological tissue samples.

Publication metadata

Author(s): Lepert G, Gouveia RM, Connon CJ, Paterson C

Publication type: Article

Publication status: Published

Journal: Faraday Discussions

Year: 2016

Volume: 187

Pages: 415-428

Print publication date: 01/06/2016

Online publication date: 06/04/2016

Acceptance date: 07/12/2015

Date deposited: 24/06/2016

ISSN (print): 1359-6640

ISSN (electronic): 1364-5498

Publisher: Royal Society of Chemistry

URL: http://dx.doi.org/10.1039/C5FD00152H

DOI: 10.1039/C5FD00152H

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