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Unraveling the multivalent binding of a marine family 6 carbohydrate-binding module with its native laminarin ligand

Lookup NU author(s): Dr Aurore Labourel


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Laminarin is an abundant brown algal storage polysaccharide. Marine microorganisms, such as Zobellia galactanivorans, produce laminarinases for its degradation, which are important for the processing of this organic matter in the ocean carbon cycle. These laminarinases are often modular, as is the case with ZgLamC which has an N-terminal GH16 module, a central family 6 carbohydrate-binding module (CBM) and a C-terminal PorSS module. To date, no studies have characterized a true marine laminarin-binding CBM6 with its natural carbohydrate ligand. The crystal structure of ZgLamC(CBM6) indicates that this CBM has two clefts for binding sugar (variable loop site, VLS; and concave face site, CFS). The ZgLamC(CBM6) VLS binds in an exo-manner and the CFS interacts in an endo-manner with laminarin. Isothermal titration calorimetry (ITC) experiments on native and mutant ZgLamC(CBM6) confirm that these binding sites have different modes of recognition for laminarin, in agreement with the regional model' postulated for CBM6-binding modules. Based on ITC data and structural data, we propose a model of ZgLamC(CBM6) interacting with different chains of laminarin in a multivalent manner, forming a complex cross-linked protein-polysaccharide network.

Publication metadata

Author(s): Jam M, Ficko-Blean E, Labourel A, Larocque R, Czjzek M, Michel G

Publication type: Article

Publication status: Published

Journal: FEBS Journal

Year: 2016

Volume: 283

Issue: 10

Pages: 1863-1879

Print publication date: 01/05/2016

Online publication date: 31/03/2016

Acceptance date: 07/11/2015

ISSN (print): 1742-464X

ISSN (electronic): 1742-4658

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1111/febs.13707


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Funder referenceFunder name
French Ministry of Higher Education and Research
French Centre National de Recherches Scientifiques (CNRS)
ANR-10-BTBR-04French National Research Agency
SAD_Obex_EMBRC 12010152Region Bretagne