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Regulation of urokinase receptor transcription by Ras- and Rho-family GTPases

Lookup NU author(s): Emel Okan, Dr Peter Jones

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Abstract

How cell adhesion is coordinated with extracellular proteolysis is a key question in understanding cell migration. Potentially, the small GTP-binding proteins that affect actin organisation and signal transduction may also regulate the expression of genes associated with extracellular proteolysis. We investigated the ability of Ras, Rac-1, Cdc42Hs, and RhoA to regulate transcription from the1.55-kb promoter region of the human urokinase plasminogen activator receptor (uPAR) gene. Constitutively active V12 H-Ras and Rho-A stimulated uPAR transcription while Cdc42Hs and Rac-1 did not. The use of Ras effector-loop mutants indicated that signalling via multiple Ras-effectors is necessary for the maximum activation of transcription. Copyright 2000 Academic Press.

Publication metadata

Author(s): Okan E; Jones P; Muller SM

Publication type: Article

Publication status: Published

Journal: Biochemical & Biophysical Research Communications

Year: 2000

Volume: 270

Issue: 3

Pages: 892-898

ISSN (print): 0006-291X

ISSN (electronic): 1090-2104

Publisher: Academic Press

URL: http://dx.doi.org/10.1006/bbrc.2000.2531

DOI: 10.1006/bbrc.2000.2531

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