Toggle Main Menu Toggle Search

Open Access padlockePrints

Routine use of probiotics in preterm infants: longitudinal impact on the microbiome and metabolome

Lookup NU author(s): Dr John Perry, Professor Nicholas EmbletonORCiD, Dr Janet Berrington, Dr Christopher StewartORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Background: Probiotics are live microbial supplements that colonize the gut and potentially exert health benefit to the host. Objectives: We aimed to determine the impact of a probiotic (Infloran (R) : Lactobacillus acidophilus-NCIMB701748 and Bifidobacterium bifidum-ATCC15696) on the bacterial and metabolic function of the preterm gut while in the neonatal intensive care unit (NICU) and following discharge. Methods: Stool samples (n = 88) were collected before, during, and after probiotic intake from 7 patients, along with time-matched controls from 3 patients. Samples were also collected following discharge home from the NICU. Samples underwent bacterial profiling analysis by 16S rRNA gene sequencing and quantitative PCR (qPCR), as well as metabolomic profiling using liquid chromatography mass spec trometry. Results: Bacterial profiling showed greater Bifidobacterium (15.1%) and Lactobacillus (4.2%) during supplementation compared to the control group (4.0% and 0%, respectively). While Lactobacillus became reduced after the probiotic had been stopped, Bifidobacterium remained high following discharge, suggestive of successful colonisation. qPCR analysis showed a significant increase (p <= 0.01) in B. bifidum in infants who received probiotic treatment compared to controls, but no significant increase was observed for L. acidophilus (p = 0.153). Metabolite profiling showed clustering based on receiving probiotic or matched controls, with distinct metabolites associated with probiotic administration. Conclusions: Probiotic species successfully colonise the preterm gut, reducing the relative abundance of potentially pathogenic bacteria, and effecting gut functioning. Bifidobacterium (but not Lactobacillus) colonised the gut in the long term, suggesting the possibility that therapeutically administered probiotics may continue to exert important functional effects on gut microbial communities in early infancy. (C) 2016 S. Karger AG, Basel

Publication metadata

Author(s): Abdulkadir B, Nelson A, Skeath T, Marrs ECL, Perry JD, Cummings SP, Embleton ND, Berrington JE, Stewart CJ

Publication type: Article

Publication status: Published

Journal: Neonatology

Year: 2016

Volume: 109

Issue: 4

Pages: 239-247

Print publication date: 01/06/2016

Online publication date: 10/02/2016

Acceptance date: 01/12/2015

ISSN (print): 1661-7800

ISSN (electronic): 1661-7819

Publisher: S. Karger AG


DOI: 10.1159/000442936


Altmetrics provided by Altmetric