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Lookup NU author(s): Monica Piatek, Dr Andreas Werner
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Natural antisense transcripts (NATs) can interfere with the expression of complementary sense transcripts with exquisite specificity. We have previously cloned NATs of Slc34a loci (encoding Na-phosphate transporters) from fish and mouse. Here we report the cloning of a human SLC34A1-related NAT that represents an alternatively spliced PFN3 transcript (Profilin3). The transcript is predominantly expressed in testis. Phylogenetic comparison suggests two distinct mechanisms producing Slc34a-related NATs: Alternative splicing of a transcript from a protein coding downstream gene (Pfn3, human/mouse) and transcription from the bi-directional promoter (Rbpja, zebrafish). Expression analysis suggested independent regulation of the complementary Slc34a mRNAs. Analysis of randomly selected bi-directionally transcribed human/mouse loci revealed limited phylogenetic conservation and independent regulation of NATs. They were reduced on X chromosomes and clustered in regions that escape inactivation. Locus structure and expression pattern suggest a NATs-associated regulatory mechanisms in testis unrelated to the physiological role of the sense transcript encoded protein. (C) 2016 The Authors. Published by Elsevier Inc.
Author(s): Piatek MJ, Henderson V, Zynad HS, Werner A
Publication type: Article
Publication status: Published
Journal: Genomics
Year: 2016
Volume: 108
Issue: 2
Pages: 56-63
Print publication date: 01/08/2016
Online publication date: 27/05/2016
Acceptance date: 25/05/2016
Date deposited: 19/10/2016
ISSN (print): 0888-7543
ISSN (electronic): 1089-8646
Publisher: Academic Press
URL: http://dx.doi.org/10.1016/j.ygeno.2016.05.004
DOI: 10.1016/j.ygeno.2016.05.004
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