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Pharmacological GSK-3beta inhibition improves osteoblast differentiation on titanium surfaces

Lookup NU author(s): Dr Silvia Mazzotta


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Rough titanium surfaces enhance the activation of Wnt canonical signaling, a pathway required for osteoblast differentiation. The present study investigated the effects of GSK3b-inhibitor (2'Z,3'E)- 6-Bromoindirubin-3'-oxime (BIO) on osteoblastic differentiation on titanium surfaces with different topography and wettability. C2C12 cells were plated on pickled, acid-etched/sand-blasted (SLA), modified hydrophilic SLA titanium discs (modSLA) and stimulated with increasing doses of BIO. Activation of Wnt canonical signaling was measured with a reporter system. Gene expression was measured in the same cell system by Real Time PCR. Osteoblastic MC3T3 cells were then plated on discs with or without BIO and the expression of osteoblast specific genes was assessed by Real Time PCR. One mM BIO activated Wnt canonical signaling in C2C12 cells on all surfaces, and the highest effect was on rough surfaces. BIO markedly increased the expression of Osteoprotegerin and Osteocalcin in MC3T3 cells on rough surfaces at the concentration of 100 nM, and on all surfaces at the concentration of 1 mM. BIO enhances Wnt signaling activation and the expression of osteoblastic genes on rough surfaces and could be a viable approach to improve cell response to implant surfaces.

Publication metadata

Author(s): Lumetti S, Ferrillo S, Mazzotta S, Macaluso GM, Bonanini M, Passeri G, Galli C

Publication type: Article

Publication status: Published

Journal: Journal of BIOLOGICAL REGULATORS & Homeostatic Agents

Year: 2014

Volume: 28

Issue: 3

Pages: 489-496

Print publication date: 01/09/2014

Acceptance date: 01/07/2014

Publisher: Biolife


PubMed id: 25316136