Browse by author
Lookup NU author(s): Dr Silvia Mazzotta
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Rough titanium surfaces enhance the activation of Wnt canonical signaling, a pathway required for osteoblast differentiation. The present study investigated the effects of GSK3b-inhibitor (2'Z,3'E)- 6-Bromoindirubin-3'-oxime (BIO) on osteoblastic differentiation on titanium surfaces with different topography and wettability. C2C12 cells were plated on pickled, acid-etched/sand-blasted (SLA), modified hydrophilic SLA titanium discs (modSLA) and stimulated with increasing doses of BIO. Activation of Wnt canonical signaling was measured with a reporter system. Gene expression was measured in the same cell system by Real Time PCR. Osteoblastic MC3T3 cells were then plated on discs with or without BIO and the expression of osteoblast specific genes was assessed by Real Time PCR. One mM BIO activated Wnt canonical signaling in C2C12 cells on all surfaces, and the highest effect was on rough surfaces. BIO markedly increased the expression of Osteoprotegerin and Osteocalcin in MC3T3 cells on rough surfaces at the concentration of 100 nM, and on all surfaces at the concentration of 1 mM. BIO enhances Wnt signaling activation and the expression of osteoblastic genes on rough surfaces and could be a viable approach to improve cell response to implant surfaces.
Author(s): Lumetti S, Ferrillo S, Mazzotta S, Macaluso GM, Bonanini M, Passeri G, Galli C
Publication type: Article
Publication status: Published
Journal: Journal of BIOLOGICAL REGULATORS & Homeostatic Agents
Year: 2014
Volume: 28
Issue: 3
Pages: 489-496
Print publication date: 01/09/2014
Acceptance date: 01/07/2014
Publisher: Biolife
URL: http://www.biolifesas.org/contentsJBRHA.htm
PubMed id: 25316136
