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Lookup NU author(s): Professor Hans-Peter Klenk
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A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500(T) (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms. (C) 2016 Elsevier Masson SAS. All rights reserved.
Author(s): Lahoum A, Aouiche A, Bouras N, Verheecke C, Klenk HP, Sabaou N, Mathieu F
Publication type: Article
Publication status: Published
Journal: Journal of Medical Mycology
Year: 2016
Volume: 26
Issue: 3
Pages: 193-200
Print publication date: 01/09/2016
Online publication date: 17/03/2016
Acceptance date: 13/02/2016
ISSN (print): 1156-5233
ISSN (electronic): 1773-0449
Publisher: Elsevier
URL: http://dx.doi.org/10.1016/j.mycmed.2016.02.017
DOI: 10.1016/j.mycmed.2016.02.017
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