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Non-covalently crosslinked chitosan nanofibrous mats prepared by electrospinning as substrates for soft tissue regeneration

Lookup NU author(s): Dr Piergiorgio GentileORCiD



This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


itosan (CS) membranes obtained by electrospinning are potentially ideal substrates for soft tissue engineering as they combine the excellent biological properties of CS with the extracellular matrix (ECM)-like structure of nanofibrous mats. However, the high amount of acid solvents required to spun CS solutions interferes with the biocompatibility of CS fibres. To overcome this limitation, a novel CS based solutions were investigated in this work. Low amount of acidic acid (0.5 M) was used and dibasic sodium phosphate (DSP) was introduced as ionic crosslinker to improve nanofibres water stability and to neutralize the acidic pH of electrospun membranes after fibres soaking in biological fluids. Randomly oriented and aligned nanofibres (118 ± 16nm size) were obtained through electrospinning process (voltage of 30 kV, 30 μL/min flow rate and temperature of 39 °C) showing mechanical properties similar to those of soft tissues (Young Modulus lower than 40 MPa in dry condition) and water stability until 7 days. C2C12 myoblast cell line was cultured on CS fibres showing that the aligned architecture of substrate induces cell orientation that can enhance skeletal muscle regeneration.

Publication metadata

Author(s): Tonda-Turo C, Ruini F, Ramella M, Boccafoschi F, Gentile P, Gioffredi E, Falvo D'Urso Labate G

Publication type: Article

Publication status: Published

Journal: Carbohydrate Polymers

Year: 2017

Volume: 162

Pages: 82-92

Print publication date: 15/04/2017

Online publication date: 15/01/2017

Acceptance date: 14/01/2017

Date deposited: 16/01/2017

ISSN (print): 0144-8617

ISSN (electronic): 1879-1344

Publisher: Elsevier


DOI: 10.1016/j.carbpol.2017.01.050


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