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Multilayer Nanoscale Encapsulation of Biofunctional Peptides to Enhance Bone Tissue Regeneration In Vivo

Lookup NU author(s): Dr Piergiorgio GentileORCiD, Dr Ana Ferreira-DuarteORCiD



This is the authors' accepted manuscript of an article that has been published in its final definitive form by Wiley - V C H Verlag GmbH & Co. KGaA, 2017.

For re-use rights please refer to the publisher's terms and conditions.


Bone tissue healing is a dynamic process that is initiated by the recruitment of osteoprogenitor cells followed by their migration, proliferation, differentiation and development of a mineralising extracellular matrix. The work aimed to manufacture a functionalised porous membrane that stimulated early events in bone healing for initiating a regenerative cascade. Layer-by-layer (LbL) assembly was proposed to modify the surface of osteoconductive electrospun meshes, based on poly(lactic-co-glycolic acid) and nanohydroxyapatite, by using poly(allylamine hydrochloride) and poly(sodium4-styrenesulfonate) as polyelectrolytes. Molecular cues were incorporated by grafting peptide fragments into the discrete nanolayers. KRSR sequence was grafted to enhance cell adhesion and proliferation, NSPVNSKIPKACCVPTELSAI to guide bone marrow mesenchymal stem cells differentiation in osteoblasts, and FHRRIKA to improve mineralisation matrix formation. Scanning electron microscopy, infrared and X-Ray photoelectron spectroscopy demonstrated the successful surface functionalisation. Furthermore, the peptides incorporation enhanced cellular processes, with good viability and significant increase of alkaline phosphatase activity, osteopontin and osteocalcin. The functionalised membrane induced a favourable in vivo response after implantation for four weeks in non-healing rat calvarial defect model. It was concluded that the multilayer nanoencapsulation of biofunctional peptides using LbL approach has significant potential as innovative manufacturing technique to improve bone regeneration in orthopaedic and craniofacial medical devices.

Publication metadata

Author(s): Gentile P, Ferreira AM, Callaghan JT, Miller CA, Atkinson J, Freeman C, Hatton PV

Publication type: Article

Publication status: Published

Journal: Advanced Healthcare Materials

Year: 2017

Volume: 6

Issue: 8

Print publication date: 01/04/2017

Online publication date: 07/02/2017

Acceptance date: 09/01/2017

Date deposited: 13/01/2017

ISSN (print): 2192-2640

ISSN (electronic): 2192-2659

Publisher: Wiley - V C H Verlag GmbH & Co. KGaA


DOI: 10.1002/adhm.201601182


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