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Lookup NU author(s): Dr Bernhard Kepplinger, Lina Mardiana, Joseph Cowell, Dr Stephanie Morton-Laing, Dr Yousef Dashti, Dr Corinne Wills, Dr John Perry, Professor Michael Goodfellow, Professor Jeff Errington, Professor Mike ProbertORCiD, Professor William Clegg, Dr Nicholas Allenby, Dr Michael HallORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
A genomic and bioactivity informed analysis of the metabolome of the extremophile Amycolatopsis sp. DEM30355 has allowed for the discovery and isolation of the polyketide antibiotic tatiomicin. Identification of the biosynthetic gene cluster was confirmed by heterologous expression in Streptomyces coelicolor M1152. Structural elucidation, including absolute stereochemical assignment, was performed using complementary crystallographic, spectroscopic and computational methods. Tatiomicin shows antibiotic activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Cytological profiling experiments suggest a putative antibiotic mode-of-action, involving membrane depolarisation and chromosomal decondensation of the target bacteria.
Author(s): Kepplinger B, Mardiana L, Cowell J, Morton-Laing S, Dashti Y, Wills C, Marrs ECL, Perry JD, Gray J, Goodfellow M, Errington J, Probert MR, Clegg W, Bogaerts J, Herrebout W, Allenby N, Hall MJ
Publication type: Article
Publication status: Published
Journal: Scientific Reports
Year: 2022
Volume: 12
Online publication date: 16/09/2022
Acceptance date: 18/08/2022
Date deposited: 06/09/2022
ISSN (electronic): 2045-2322
Publisher: Nature Publishing Group
URL: https://doi.org/10.1038/s41598-022-18726-3
DOI: 10.1038/s41598-022-18726-3
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