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The Role of Kv1.2 Channel in Electrotaxis Cell Migration

Lookup NU author(s): Dr Seva TelezhkinORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Voltage-gated potassium Kv1.2 channels play pivotal role in maintaining of resting membrane potential and, consequently, regulation of cellular excitability of neurons. Endogenously generated electric field (EF) have been proven as an important regulator for cell migration and tissue repair. The mechanisms of ion channel involvement in EF-induced cell responses are extensively studied but largely are poorly understood. In this study we generated three COS-7 clones with different expression levels of Kv1.2 channel, and confirmed their functional variations with patch clamp analysis. Time-lapse imaging analysis showed that EF-induced cell migration response was Kv1.2 channel expression level depended. Inhibition of Kv1.2 channels with charybdotoxin (ChTX) constrained the sensitivity of COS-7 cells to EF stimulation more than their motility. Immunocytochemistry and pull-down analyses demonstrated association of Kv1.2 channels with actin-binding protein cortactin and its re-localization to the cathode-facing membrane at EF stimulation, which confirms the mechanism of EF-induced directional migration. This study displays that Kv1.2 channels represent an important physiological link in EF-induced cell migration. The described mechanism suggests a potential application of EF which may improve therapeutic performance in curing injuries of neuronal and/or cardiac tissue repair, post operational therapy, and various degenerative syndromes.

Publication metadata

Author(s): Zhang G, Edmundson M, Telezhkin V, Gu Y, Wei X, Kemp PJ, Song B

Publication type: Article

Publication status: Published

Journal: Journal of Cellular Physiology

Year: 2016

Volume: 231

Issue: 6

Pages: 1375–1384

Print publication date: 01/06/2016

Online publication date: 18/11/2015

Acceptance date: 17/11/2015

Date deposited: 09/03/2017

ISSN (print): 0021-9541

ISSN (electronic): 1097-4652

Publisher: Wiley


DOI: 10.1002/jcp.25259

PubMed id: 26580832


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