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Lookup NU author(s): Dr Seva TelezhkinORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Cellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. Transplanted Dental Pulp Stem/Progenitor Cells (DPSCs) have demonstrated early promise in experimental models of spinal cord injury and stroke, despite limited evidence of neuronal and glial-like differentiation after transplantation. Here, we report, for the first time, on the ability of single cell-derived clonal cultures of murine DPSCs to differentiate in vitro into immature neuronal-like and oligodendrocyte-like cells. Importantly, only DPSC clones with high nestin mRNA expression levels were found to successfully differentiate into Map2 and NF-positive neuronal-like cells. Neuronally differentiated DPSCs possessed a membrane capacitance comparable with primary cultured striatal neurons and small inward voltage-activated K(+) but not outward Na(+) currents were recorded suggesting a functionally immature phenotype. Similarly, only high nestin-expressing clones demonstrated the ability to adopt Olig1, Olig2, and MBP-positive immature oligodendrocyte-like phenotype. Together, these results demonstrate that appropriate markers may be used to provide an early indication of the suitability of a cell population for purposes where differentiation into a specific lineage may be beneficial and highlight that further understanding of heterogeneity within mixed cellular populations is required.
Author(s): Young F, Telezhkin V, Youde SJ, Langley MS, Stack M, Kemp PJ, Waddington RJ, Sloan AJ, Song B
Publication type: Article
Publication status: Published
Journal: Stem Cells International
Year: 2016
Volume: 2016
Print publication date: 09/05/2016
Acceptance date: 08/05/2016
Date deposited: 09/03/2017
ISSN (print): 1687-966X
ISSN (electronic): 1687-9678
Publisher: Hindawi
URL: https://doi.org/10.1155/2016/1290561
DOI: 10.1155/2016/1290561
PubMed id: 27313623
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