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Planomonospora algeriensis sp nov., an actinobacterium isolated from a Saharan soil of Algeria

Lookup NU author(s): Professor Hans-Peter Klenk

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Abstract

A filamentous actinobacterium, designated strain PM3(T), was isolated from a Saharan soil sample collected from B,ni-AbbSs, B,char (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3(T). The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso-diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H-2) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be C-17:1 omega 9c (38.6%) and C-17:0 (24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3(T) shared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632(T) (99.3%), Planomonospora parontospora subsp. parontospora DSM 43177(T) (99.2%) and P. parontospora subsp. antibiotica DSM 43869(T) (99.0%). DNA-DNA hybridization values between strain PM3(T) and the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3(T) represents a novel species of the genus Planomonospora, for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3(T) (=DSM 46752(T) = CECT 9047(T)).

Publication metadata

Author(s): Chaouch FC, Bouras N, Mokrane S, Bouznada K, Zitouni A, Potter G, Sproer C, Klenk HP, Sabaou N

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2017

Volume: 110

Issue: 2

Pages: 245-252

Print publication date: 01/02/2017

Online publication date: 01/11/2016

Acceptance date: 22/10/2016

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer

URL: http://doi.org/10.1007/s10482-016-0795-1

DOI: 10.1007/s10482-016-0795-1

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