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Nakamurella silvestris sp nov., an actinobacterium isolated from alpine forest soil

Lookup NU author(s): Dr Imen Nouioui, Professor Hans-Peter Klenk


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A Gram-stain-positive, non-motile, catalase-positive and cytochrome c oxidase-negative bacterium, designated strain S20-107(T), was isolated from alpine forest soil. Growth occurred at 0-30 degrees C, at pH 6-9 and in the presence of 0-3% (w/v) NaCI. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S20-107T was related to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity with Nakamurella flavida DS-52(T) (96.1%). Strain S20-107(T) showed <96.1% 16S rRNA gene sequence similarities to all other recognized members of the genus Nakamurella. The cell-wall peptidoglycan contained meso-diaminopinnelic acid. The major whole-cell sugars were glucose, galactose, mannose, arabinose, ribose and rhamnose. The strain contained MK-8(H-4) as the predominant menaquinone and diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminophospholipid as the major polar lipids. The major cellular fatty acids were anteiso-C-15:0, C-16:0, summed feature 3 (C-16:1 omega 6c and/or C-16:(1)omega 7c and/or iso-C-15:0 2-0H) and iso-C-16:0. The genomic DNA G+C content was 70.5 mol%. Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-107(T) represents a novel species of the genus Nakamurella, for which the name Nakamurella silvestris sp. nov. is proposed. The type strain is S20-107(T) (=DSM 102309(T)=LMG 29427(T)).

Publication metadata

Author(s): Franca L, Albuquerque L, Zhang DC, Nouioui I, Klenk HP, da Costa MS, Margesin R

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2016

Volume: 66

Pages: 5460-5464

Print publication date: 01/12/2016

Online publication date: 01/12/2016

Acceptance date: 03/10/2016

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: The Microbiology Society


DOI: 10.1099/ijsem.0.001541


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Funder referenceFunder name
FEDER funds through the Operational Programme Competitiveness Factors - COMPETE
15/40.3Autonomous Province of Bozen/Bolzano, South Tyrol, Promotion of Educational Policies, University and Research Department
UID/NEU/04539/2013FCT-Foundation for Science and Technology