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The design and structural characterization of a synthetic pentatricopeptide repeat protein

Lookup NU author(s): Dr Will Stanley

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Abstract

© 2015.Proteins of the pentatricopeptide repeat (PPR) superfamily are characterized by tandem arrays of a degenerate 35-amino-acid -hairpin motif. PPR proteins are typically single-stranded RNA-binding proteins with essential roles in organelle biogenesis, RNA editing and mRNA maturation. A modular, predictable code for sequence-specific binding of RNA by PPR proteins has recently been revealed, which opens the door to the de novo design of bespoke proteins with specific RNA targets, with widespread biotechnological potential. Here, the design and production of a synthetic PPR protein based on a consensus sequence and the determination of its crystal structure to 2.2Å resolution are described. The crystal structure displays helical disorder, resulting in electron density representing an infinite superhelical PPR protein. A structural comparison with related tetratricopeptide repeat (TPR) proteins, and with native PPR proteins, reveals key roles for conserved residues in directing the structure and function of PPR proteins. The designed proteins have high solubility and thermal stability, and can form long tracts of PPR repeats. Thus, consensus-sequence synthetic PPR proteins could provide a suitable backbone for the design of bespoke RNA-binding proteins with the potential for high specificity.


Publication metadata

Author(s): Gully BS, Shah KR, Lee M, Shearston K, Smith NM, Sadowska A, Blythe AJ, Bernath-Levin K, Stanley WA, Small ID, Bond CS

Publication type: Article

Publication status: Published

Journal: Acta Crystallographica Section D: Biological Crystallography

Year: 2015

Volume: 71

Issue: Part 2

Pages: 196-208

Print publication date: 01/02/2015

Online publication date: 23/01/2015

Acceptance date: 12/11/2014

ISSN (print): 0907-4449

ISSN (electronic): 2059-7983

Publisher: International Union of Crystallography

URL: https://doi.org/10.1107/S1399004714024869

DOI: 10.1107/S1399004714024869

PubMed id: 25664731


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