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Porphyromonas gingivalis lipopolysaccharide delays human polymorphonuclear leukocyte apoptosis in vitro

Lookup NU author(s): Professor Philip Preshaw


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Apoptosis (programmed cell death) is a mechanism by which superfluous or damaged cells undergo changes that lead to selective removal from organ systems by phagocytic cells. Certain bacterial products delay apoptosis in neutrophils (PMNs). In this study, PMNs were incubated for up to 8 h with varying concentrations of lipopolysaccharide (LPS), lipid A or capsular polysaccharide isolated from 3 strains of Porphyromonas gingivalis (Pg) (strains HG-184, A7A1-28 and 381). Assay runs included controls containing cells and medium but no bacterial products. Fluorescence microscopy was used to evaluate apoptotic changes. PMNs exhibited a time-dependent increase in the number of apoptotic cells. When cells were cultured in the presence of LPS from any of the 3 Pg strains, apoptosis was delayed in a dose-dependent fashion (p < 0.05). The effects of these LPS preparations were similar to each other and to Escherichia coli 0111:B4 LPS. Lipid A from the 3 Pg strains also delayed apoptosis (p < 0.05), but was less potent than LPS or synthetic lipid A. Capsular polysaccharide had no significant effect on apoptosis (p > 0.05). Thus, LPS and lipid A from P. gingivalis appear to modulate the functional lifespan of PMNs. This could potentiate the inflammatory and destructive components of periodontal diseases.

Publication metadata

Author(s): Preshaw PM, Schifferle RE, Walters JD

Publication type: Article

Publication status: Published

Journal: Journal of Periodontal Research

Year: 1999

Volume: 34

Issue: 4

Pages: 197-202

Print publication date: 01/05/1999

ISSN (print): 0022-3484

ISSN (electronic): 1600-0765

Publisher: Wiley-Blackwell Publishing, Inc.


DOI: 10.1111/j.1600-0765.1999.tb02242.x

Notes: Denmark 0022-3484


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