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Single-cell analysis of mitochondrial DNA

Lookup NU author(s): Dr Brendan PayneORCiD, Dr Lynsey Cree, Professor Patrick Chinnery


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© Springer Science+Business Media New York 2015. All right reserved. Understanding the biology of mitochondrial DNA (mtDNA) at the single-cell level has yielded important insights into inheritance, disease, and normal aging. In nuclear gene disorders of mtDNA maintenance, neurodegeneration, and aging, different somatic mtDNA mutations exist within individual cells and may be missed by techniques applied to whole tissue DNA extract. We therefore provide a method for characterizing mtDNA within single skeletal muscle fibers. During embryogenesis, mtDNA content is subject to a tight bottleneck and this may account for differential segregation of mutant mtDNA in offspring. We also present a method to study this phenomenon by single-cell analysis of embryonic PGCs (primordial germ cells).

Publication metadata

Author(s): Payne BAI, Cree L, Chinnery PF

Editor(s): Weissig V; Edeas M

Publication type: Book Chapter

Publication status: Published

Book Title: Mitochondrial Medicine

Year: 2015

Volume: 1264

Pages: 67-76

Print publication date: 29/01/2015

Online publication date: 06/01/2015

Acceptance date: 01/01/1900

Series Title: Methods in Molecular Biology

Publisher: Springer New York

Place Published: New York, NY, USA


DOI: 10.1007/978-1-4939-2257-4_7

Notes: 9781493922567 Hardback ISBN

Library holdings: Search Newcastle University Library for this item

ISBN: 9781493922574