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Lookup NU author(s): Dr Imen Nouioui, Professor Hans-Peter Klenk, Dr Maria Del Carmen Montero-CalasanzORCiD
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© 2016, Springer International Publishing Switzerland. The taxonomic position of an actinobacterial isolate, designated strain BMG 822T, isolated from limestone from the Amphitheater of El Jem (Coliseum Thysdrus), Tunisia, was established using a polyphasic approach. Strain BMG 822T was found to grow well at 30 °C and pH 6.5–8.0, and to be coral-coloured, Gram-positive, catalase and oxidase negative. Whole cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, glucose, galactose and ribose. The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, an unidentified glycophospholipid and six unidentified phospholipids. MK-9(H4) was found to be the predominant menaquinone, followed by MK-9(H2) and MK-9. The major cellular fatty acids were identified as iso-C16:0, C18:1ω9c, C17:1ω8c and iso-H-C16:1. The G+C content of the DNA (73.2%) is typical of the genus. High degrees of 16S rRNA gene sequence similarity were found with the type strains of the genus Blastococcus (97.1–98.3%) followed by the type strains of Modestobacter (96.8–97.8%). Based on the above data and the phenotypic differences from the type strains of Blastococcus species, it is proposed that the isolate BMG 822T (=DSM 46837T=CECT 8823T) should be classified as the type strain of a novel species, Blastococcus colisei sp. nov.
Author(s): Hezbri K, Nouioui I, Rohde M, Schumann P, Gtari M, Klenk H-P, Montero-Calasanz MC, Ghodhbane-Gtari F
Publication type: Article
Publication status: Published
Journal: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Year: 2017
Volume: 110
Issue: 3
Pages: 339-346
Print publication date: 01/03/2017
Online publication date: 28/11/2016
Acceptance date: 10/11/2016
ISSN (print): 0003-6072
ISSN (electronic): 1572-9699
Publisher: Springer Netherlands
URL: https://doi.org/10.1007/s10482-016-0804-4
DOI: 10.1007/s10482-016-0804-4
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