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Frankia discariae sp. nov.: an infective and effective microsymbiont isolated from the root nodule of Discaria trinervis

Lookup NU author(s): Dr Imen Nouioui, Dr Maria Del Carmen Montero-CalasanzORCiD, Professor Hans-Peter Klenk

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Abstract

© 2017 Springer-Verlag Berlin Heidelberg Strain BCU110501T was the first isolate reported to fulfill Koch’s postulates by inducing effective nodules on its host plant of origin Discaria trinervis (Rhalmnaceae). Based on 16S rRNA gene sequence similarities, the strain was found to be most closely related to the type strain of Frankia elaeagni DSM 46783T (98.6%) followed by F. alni DSM 45986T (98.2%), F. casuarinae DSM 45818T (97.8%) and F. inefficacies DSM 45817T (97.8%). Digital DNA:DNA hybridizations (dDDH) between strain BCU110501Tand the type strains of other Frankia species were clearly below the cutoff point of 70%. The G+C content of DNA is 72.36%. The cell wall of strain BCU110501T contained meso-diaminopimelic acid and the cell sugars were galactose, glucose, mannose, xylose and ribose. Polar lipids were phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipid (GPL1−3), phosphatidylglycerol (PG) and an unknown lipid (L). The major fatty acids of strain BCU110501T consisted of iso-C16:0, C17:1 w8c and C16:0. Major menaquinones were MK9 (H4), MK9 (H6) and MK9 (H2). Based on these analyses, strain BCU110501T (=DSM 46785T=CECT 9042T) should be classified as the type strain of a novel Frankia species, for which the name Frankia discariae sp. nov. is proposed.

Publication metadata

Author(s): Nouioui I, Del Carmen Montero-Calasanz M, Ghodhbane-Gtari F, Rohde M, Tisa LS, Klenk H-P, Gtari M

Publication type: Article

Publication status: Published

Journal: Archives of Microbiology

Year: 2017

Volume: 199

Issue: 5

Pages: 641-647

Print publication date: 01/07/2017

Online publication date: 19/01/2017

Acceptance date: 04/01/2017

ISSN (print): 0302-8933

ISSN (electronic): 1432-072X

Publisher: Springer Verlag

URL: https://doi.org/10.1007/s00203-017-1337-6

DOI: 10.1007/s00203-017-1337-6

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