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Lookup NU author(s): Dr Adrien Vigneron,
Professor Ian Head
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
The Pennsylvania region hosts numerous oil and gas reservoirs and the presence of hydrocarbons in groundwater has been locally observed. However, these methane-containing freshwater ecosystems remain poorly explored despite their potential importance in the carbon cycle. Methane isotope analysis and analysis of low molecular weight hydrocarbon gases from 18 water wells indicated that active methane cycling may be occurring in methane-containing groundwater from the Pennsylvania region. Consistent with this observation, multigenic qPCR and gene sequencing (16S rRNA genes, mcrA, and pmoA genes) indicated abundant populations of methanogens, ANME-2d (average of 1.54 × 104 mcrA gene per milliliter of water) and bacteria associated with methane oxidation (NC10, aerobic methanotrophs, methylotrophs; average of 2.52 × 103 pmoA gene per milliliter of water). Methane cycling therefore likely represents an important process in these hydrocarbon-containing aquifers. The microbial taxa and functional genes identified and geochemical data suggested that (i) methane present is at least in part due to methanogens identified in situ; (ii) Potential for aerobic and anaerobic methane oxidation is important in groundwater with the presence of lineages associated with both anaerobic an aerobic methanotrophy; (iii) the dominant methane oxidation process (aerobic or anaerobic) can vary according to prevailing conditions (oxic or anoxic) in the aquifers; (iv) the methane cycle is closely associated with the nitrogen cycle in groundwater methane seeps with methane and/or methanol oxidation coupled to denitrification or nitrate and nitrite reduction.
Author(s): Vigneron A, Bishop A, Alsop EB, Hull K, Rhodes I, Hendricks R, Head IM, Tsesmetzis N
Publication type: Article
Publication status: Published
Journal: Frontiers in Microbiology
Online publication date: 05/04/2017
Acceptance date: 22/03/2017
Date deposited: 25/04/2017
ISSN (electronic): 1664-302X
Publisher: Frontiers Research Foundation
PubMed id: PMC5380731
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