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Generation of recombinant N-linked glycoproteins in E. coli

Lookup NU author(s): Professor Phillip WrightORCiD

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Abstract

© 2017, Springer Science+Business Media LLC. The production of N-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, Escherichia coli. This methods chapter will outline the components and procedures needed to produce N-linked glycoproteins in E. coli, utilizing Campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. To ensure a successful outcome, various methods will be highlighted that can confirm glycoprotein production to a high degree of confidence, including the gold standard of mass spectrometry analysis.

Publication metadata

Author(s): Strutton B, Jaffe SRP, Pandhal J, Wright PC

Publication type: Book Chapter

Publication status: Published

Book Title: Methods in Molecular Biology

Year: 2017

Volume: 1586

Pages: 233-250

Online publication date: 04/05/2017

Acceptance date: 02/04/2016

Series Title: Methods in Molecular Biology

Publisher: Humana Press Inc.

URL: https://doi.org/10.1007/978-1-4939-6887-9_15

DOI: 10.1007/978-1-4939-6887-9_15

Library holdings: Search Newcastle University Library for this item

ISBN: 9781493968879

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