Protocols for investigating the microbial communities of oil and gas reservoirs

Tsesmetzis, N; Maguire, MJ; Head, IM; Lomans, BP

doi:10.1007/8623_2016_212

Protocols for investigating the microbial communities of oil and gas reservoirs

Lookup NU author(s): Professor Nicolas Tsesmetzis, Professor Ian Head

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Abstract

Recent studies have shown that microorganisms and microbial activity in oil reservoirs and the associated production systems are much more prominent than was originally thought. These findings, in conjunction with technological advances in bio-related disciplines, have revolutionized the way we understand and manage these biological processes.Here we present a series of protocols outlining the best practices for handling core and produced fluid material from petroleum reservoirs for isolation of nucleic acids, microbial profiling, and whole metagenome sequencing.