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Lookup NU author(s): Ayman Alzu'bi, Emerita Professor Susan Lindsay, Lauren Harkin, Dr Steven LisgoORCiD, Dr Gavin ClowryORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
In human telencephalon at 8–12 postconceptional weeks, ribonucleic acid quantitative sequencing andimmunohistochemistry revealed cortical chicken ovalbumin upstream promotor-transcription factor 1 (COUP-TFI)expression in a high ventro-posterior to low anterior gradient except for raised immunoreactivity in the anterior ventralpallium. Unlike in mouse, COUP-TFI and SP8 were extensively co-expressed in dorsal sensory neocortex and dorsalhippocampus whereas COUPTFI/COUPTFII co-expression defined ventral temporal cortex and ventral hippocampus. In theganglionic eminences (GEs) COUP-TFI immunoreactivity demarcated the proliferative zones of caudal GE (CGE), dorsalmedial GE (MGE), MGE/lateral GE (LGE) boundary, and ventral LGE whereas COUP-TFII was limited to ventral CGE and theMGE/LGE boundary. Co-labeling with gamma amino butyric acidergic interneuron markers revealed that COUP-TFI wasexpressed in subpopulations of either MGE-derived (SOX6+) or CGE-derived (calretinin+/SP8+) interneurons. COUP-TFII wasmainly confined to CGE-derived interneurons. Twice as many GAD67+ cortical cells co-labeled for COUP-TFI than for COUPTFII.A fifth of COUP-TFI cells also co-expressed COUP-TFII, and cells expressing either transcription factor followedposterior or anterio-lateral pathways into the cortex, therefore, a segregation of migration pathways according to COUP-TFexpression as proposed in mouse was not observed. In cultures differentiated from isolated human cortical progenitors,many cells expressed either COUP-TF and 30% also co-expressed GABA, however no cells expressed NKX2.1. This suggestsinterneurons could be generated intracortically from progenitors expressing either COUP-TF.
Author(s): Alzu'bi A, Lindsay SJ, Harkin LF, McIntyre J, Lisgo SN, Clowry GJ
Publication type: Article
Publication status: Published
Journal: Cerebral Cortex
Year: 2017
Volume: 27
Issue: 10
Pages: 4971-4987
Print publication date: 01/10/2017
Online publication date: 09/08/2017
Acceptance date: 03/07/2017
Date deposited: 07/09/2017
ISSN (print): 1047-3211
ISSN (electronic): 1460-2199
Publisher: Oxford University Press
URL: https://doi.org/10.1093/cercor/bhx185
DOI: 10.1093/cercor/bhx185
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