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Soluble and immobilized graphene oxide activates complement system differently dependent on surface oxidation state

Lookup NU author(s): Professor Moein MoghimiORCiD


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© 2015 Elsevier Ltd. Graphene oxide (GO) is believed to become applicable in biomedical products and medicine, thereby necessitating appropriate safety evaluation dependent on their applications and the route of administration. We have examined the effect of GO form (in solution versus immobilized) and oxidation state on two related elements of innate immunity: the complement system and interleukin-6 (IL-6) release in human blood. In solution, there was a decrease in GO-mediated complement activation with decreasing surface oxygen content (and altered oxygen functionality), whereas with immobilized GO complement response were reversed and increased with decreasing oxygen content. GO solutions, at concentrations below complement activating threshold, did not induce IL-6 release from human blood leukocytes, and further dampened lipopolysaccharide-induced IL-6 release in the whole blood. The latter effect became more profound with GO's having higher oxygen content. This protective role of GO solutions, however, disappeared at higher concentrations above complement-activating threshold. We discuss these results in relation to GO surface structure and properties, and implications for local administration and development of GO-based implantable devices.

Publication metadata

Author(s): Wibroe PP, Petersen SV, Bovet N, Laursen BW, Moghimi SM

Publication type: Article

Publication status: Published

Journal: Biomaterials

Year: 2016

Volume: 78

Pages: 20-26

Print publication date: 01/02/2016

Online publication date: 19/11/2015

Acceptance date: 17/11/2015

ISSN (print): 0142-9612

ISSN (electronic): 1878-5905

Publisher: Elsevier Ltd


DOI: 10.1016/j.biomaterials.2015.11.028

PubMed id: 26646624


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