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Lookup NU author(s): Dr Mike Althaus
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Amiloride is a widely used diuretic that blocks epithelial sodium channels (ENaCs). These heterotrimeric transmembrane proteins, assembled from β, γ and α or δ subunits, effectively control water transport across epithelia and sodium influx into non-epithelial cells. The functional role of δβγENaC in various organs, including the human brain, is still poorly understood and no pharmacological tools are available for the functional differentiation between α- and δ-containing ENaCs. Here we report several photoswitchable versions of amiloride. One compound, termed PA1, enables the optical control of ENaC channels, in particular the δβγ isoform, by switching between blue and green light, or by turning on and off blue light. PA1 was used to modify functionally δβγENaC in amphibian and mammalian cells. We also show that PA1 can be used to differentiate between δβγENaC and αβγENaC in a model for the human lung epithelium.
Author(s): Schönberger M, Althaus M, Fronius M, Clauss W, Trauner D
Publication type: Article
Publication status: Published
Journal: Nature Chemistry
Print publication date: 31/08/2014
Online publication date: 20/07/2014
Acceptance date: 12/06/2014
ISSN (print): 1755-4330
ISSN (electronic): 1755-4349
Publisher: Nature Publishing Group
PubMed id: 25054942
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