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Heterogeneity in pure microbial systems: Experimental measurements and modeling

Lookup NU author(s): Dr Rebeca Gonzalez-Cabaleiro, Anca Mitchell, Dr Wendy Smith, Professor Anil Wipat, Dr Dana OfiteruORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2017 González-Cabaleiro, Mitchell, Smith, Wipat and Ofiteru. Cellular heterogeneity influences bioprocess performance in ways that until date are not completely elucidated. In order to account for this phenomenon in the design and operation of bioprocesses, reliable analytical and mathematical descriptions are required. We present an overview of the single cell analysis, and the mathematical modeling frameworks that have potential to be used in bioprocess control and optimization, in particular for microbial processes. In order to be suitable for bioprocess monitoring, experimental methods need to be high throughput and to require relatively short processing time. One such method used successfully under dynamic conditions is flow cytometry. Population balance and individual based models are suitable modeling options, the latter one having in particular a good potential to integrate the various data collected through experimentation. This will be highly beneficial for appropriate process design and scale up as a more rigorous approach may prevent a priori unwanted performance losses. It will also help progressing synthetic biology applications to industrial scale.

Publication metadata

Author(s): Gonzalez-Cabaleiro R, Mitchell AM, Smith W, Wipat A, Ofiteru ID

Publication type: Review

Publication status: Published

Journal: Frontiers in Microbiology

Year: 2017

Volume: 8

Online publication date: 20/09/2017

Acceptance date: 05/09/2017

ISSN (electronic): 1664-302X

Publisher: Frontiers Media S.A.


DOI: 10.3389/fmicb.2017.01813