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Lookup NU author(s): Dr Subinoy Rana
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Supramolecular complexes of a family of positively charged conjugated polymers (CPs) and green fluorescent protein (GFP) create a fluorescence resonance energy transfer (FRET)-based ratiometric biosensor array. Selective multivalent interactions of the CPs with mammalian cell surfaces caused differential change in FRET signals, providing a fingerprint signature for each cell type. The resulting fluorescence signatures allowed the identification of 16 different cell types and discrimination between healthy, cancerous, and metastatic cells, with the same genetic background. While the CP-GFP sensor array completely differentiated between the cell types, only partial classification was achieved for the CPs alone, validating the effectiveness of the ratiometric sensor. The utility of the biosensor was further demonstrated in the detection of blinded unknown samples, where 121 of 128 samples were correctly identified. Notably, this selectivity-based sensor stratified diverse cell types in minutes, using only 2000 cells, without requiring specific biomarkers or cell labeling.
Author(s): Rana S, Elci SG, Mout R, Singla AK, Yazdani M, Bendur M, Bajaj A, Saha K, Bunz UHF, Jirik FR, Rotello VM
Publication type: Article
Publication status: Published
Journal: Journal of the American Chemical Society
Year: 2016
Volume: 138
Pages: 4522-4529
Print publication date: 06/04/2016
Online publication date: 11/03/2016
Acceptance date: 11/03/2016
ISSN (print): 0002-7863
ISSN (electronic): 1520-5126
Publisher: American Chemical Society
URL: https://doi.org/10.1021/jacs.6b00067
DOI: 10.1021/jacs.6b00067
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