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Ratiometric array of conjugated polymers−fluorescent protein provides a robust mammalian cell sensor

Lookup NU author(s): Dr Subinoy Rana

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Abstract

Supramolecular complexes of a family of positively charged conjugated polymers (CPs) and green fluorescent protein (GFP) create a fluorescence resonance energy transfer (FRET)-based ratiometric biosensor array. Selective multivalent interactions of the CPs with mammalian cell surfaces caused differential change in FRET signals, providing a fingerprint signature for each cell type. The resulting fluorescence signatures allowed the identification of 16 different cell types and discrimination between healthy, cancerous, and metastatic cells, with the same genetic background. While the CP-GFP sensor array completely differentiated between the cell types, only partial classification was achieved for the CPs alone, validating the effectiveness of the ratiometric sensor. The utility of the biosensor was further demonstrated in the detection of blinded unknown samples, where 121 of 128 samples were correctly identified. Notably, this selectivity-based sensor stratified diverse cell types in minutes, using only 2000 cells, without requiring specific biomarkers or cell labeling.


Publication metadata

Author(s): Rana S, Elci SG, Mout R, Singla AK, Yazdani M, Bendur M, Bajaj A, Saha K, Bunz UHF, Jirik FR, Rotello VM

Publication type: Article

Publication status: Published

Journal: Journal of the American Chemical Society

Year: 2016

Volume: 138

Pages: 4522-4529

Print publication date: 06/04/2016

Online publication date: 11/03/2016

Acceptance date: 11/03/2016

ISSN (print): 0002-7863

ISSN (electronic): 1520-5126

Publisher: American Chemical Society

URL: https://doi.org/10.1021/jacs.6b00067

DOI: 10.1021/jacs.6b00067


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