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Lookup NU author(s): Dr Subinoy Rana
This is the authors' accepted manuscript of a letter published in its final definitive form in 2015. For re-use rights please refer to the publishers terms and conditions.
Screening methods that use traditional genomic, transcriptional, proteomic and metabonomic signatures to characterize drug mechanisms are known. However, they are time consuming and require specialized equipment. Here, we present a high-throughput multichannel sensor platform that can profile the mechanisms of various chemotherapeutic drugs in minutes. The sensor consists of a gold nanoparticle complexed with three different fluorescent proteins that can sense drug-induced physicochemical changes on cell surfaces. In the presence of cells, fluorescent proteins are rapidly displaced from the gold nanoparticle surface and fluorescence is restored. Fluorescence ‘turn on’ of the fluorescent proteins depends on the drug-induced cell surface changes, generating patterns that identify specific mechanisms of cell death induced by drugs. The nanosensor is generalizable to different cell types and does not require processing steps before analysis, offering an effective way to expedite research in drug discovery, toxicology and cell-based sensing.
Author(s): Rana S, Le NDB, Mout R, Saha K, Tonga GY, Bain RES, Miranda OR, Rotello CM, Rotello VM
Publication type: Letter
Publication status: Published
Journal: Nature Nanotechnology
Year: 2015
Volume: 10
Pages: 65-69
Online publication date: 15/12/2014
Acceptance date: 04/11/2014
ISSN (print): 1748-3387
ISSN (electronic): 1748-3395
URL: https://doi.org/10.1038/nnano.2014.285
DOI: 10.1038/nnano.2014.285
