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Analysis of the Pho regulon in Streptomyces tsukubaensis

Lookup NU author(s): Dr Fernando Santos Beneit

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Abstract

© 2017 Elsevier GmbH Phosphate regulation of antibiotic biosynthesis in Streptomyces has been studied due to the importance of this genus as a source of secondary metabolites with biological activity. Streptomyces tsukubaensis is the main producer of tacrolimus (or FK506), an immunosuppressant macrolide that generates important benefits for the pharmaceutical market. However, the production of tacrolimus is under a negative control by phosphate and, therefore, is important to know the molecular mechanism of this regulation. Despite its important role, there are no reports about the Pho regulon in S. tsukubaensis. In this work we combined transcriptional studies on the response to phosphate starvation with the search for PHO boxes in the whole genome sequence of S. tsukubaensis. As a result, we identified a set of genes responding to phosphate starvation and containing PHO boxes that include common Pho regulon members but also new species-specific candidates. In addition, we demonstrate for the first time the functional activity of PhoP from S. tsukubaensis through complementation studies in a Streptomyces coelicolor ΔphoP strain. For this purpose, we developed an anhydrotetracycline inducible system that can be applied to the controlled expression of target genes.

Publication metadata

Author(s): Ordóñez-Robles M, Santos-Beneit F, Rodríguez-García A, Martín JF

Publication type: Article

Publication status: Published

Journal: Microbiological Research

Year: 2017

Volume: 205

Pages: 80-87

Print publication date: 01/12/2017

Online publication date: 24/08/2017

Acceptance date: 19/08/2017

ISSN (print): 0944-5013

Publisher: Elsevier GmbH

URL: https://doi.org/10.1016/j.micres.2017.08.010

DOI: 10.1016/j.micres.2017.08.010

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