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Lookup NU author(s): Dr Keng Wooi Ng,
Dr Wing Man Lau
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer, 2015.
For re-use rights please refer to the publisher's terms and conditions.
Immunodiagnostic microneedles provide a novel way to extract protein biomarkers from the skin in a minimally invasive manner for analysis in vitro. The technology could overcome challenges in biomarker analysis specifically in solid tissue, which currently often involves invasive biopsies. This study describes the development of a multiplex immunodiagnostic device incorporating mechanisms to detect multiple antigens simultaneously, as well as internal assay controls for result validation. A novel detection method is also proposed. It enables signal detection specifically at microneedle tips and therefore may aid the construction of depth profiles of skin biomarkers. The detection method can be coupled with computerised densitometry for signal quantitation. The antigen specificity, sensitivity and functional stability of the device were assessed against a number of model biomarkers. Detection and analysis of endogenous antigens (interleukins 1α and 6) from the skin using the device was demonstrated. The results were verified using conventional enzyme-linked immunosorbent assays. The detection limit of the microneedle device, at ≤10 pg/mL, was at least comparable to conventional plate-based solid-phase enzyme immunoassays.
Author(s): Ng KW, Lau WM, Williams AC
Publication type: Article
Publication status: Published
Journal: Drug Delivery and Translational Research
Print publication date: 01/08/2015
Online publication date: 05/05/2015
Acceptance date: 01/05/2015
Date deposited: 11/06/2019
ISSN (print): 2190-393X
ISSN (electronic): 2190-3948
PubMed id: 25939431
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