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Towards pain-free diagnosis of skin diseases through multiplexed microneedles: biomarker extraction and detection using a highly sensitive blotting method

Lookup NU author(s): Dr Keng Wooi Ng, Dr Wing Man Lau

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer, 2015.

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Abstract

Immunodiagnostic microneedles provide a novel way to extract protein biomarkers from the skin in a minimally invasive manner for analysis in vitro. The technology could overcome challenges in biomarker analysis specifically in solid tissue, which currently often involves invasive biopsies. This study describes the development of a multiplex immunodiagnostic device incorporating mechanisms to detect multiple antigens simultaneously, as well as internal assay controls for result validation. A novel detection method is also proposed. It enables signal detection specifically at microneedle tips and therefore may aid the construction of depth profiles of skin biomarkers. The detection method can be coupled with computerised densitometry for signal quantitation. The antigen specificity, sensitivity and functional stability of the device were assessed against a number of model biomarkers. Detection and analysis of endogenous antigens (interleukins 1α and 6) from the skin using the device was demonstrated. The results were verified using conventional enzyme-linked immunosorbent assays. The detection limit of the microneedle device, at ≤10 pg/mL, was at least comparable to conventional plate-based solid-phase enzyme immunoassays.


Publication metadata

Author(s): Ng KW, Lau WM, Williams AC

Publication type: Article

Publication status: Published

Journal: Drug Delivery and Translational Research

Year: 2015

Volume: 5

Issue: 4

Pages: 387–396

Print publication date: 01/08/2015

Online publication date: 05/05/2015

Acceptance date: 01/05/2015

Date deposited: 11/06/2019

ISSN (print): 2190-393X

ISSN (electronic): 2190-3948

Publisher: Springer

URL: https://doi.org/10.1007/s13346-015-0231-5

DOI: 10.1007/s13346-015-0231-5

PubMed id: 25939431


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