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ζ potential changing nanoparticles as cystic fibrosis transmembrane conductance regulator gene delivery system: An in vitro evaluation

Lookup NU author(s): Dr Matt WilcoxORCiD, Dr Peter Chater, Professor Jeffrey Pearson

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Abstract

© 2017 Future Medicine Ltd. Aim: Aim of the study was the development of ζ potential changing nanoparticles as gene delivery system for the cystic fibrosis transmembrane conductance regulator gene. Methods: Chitosan and carboxymethyl cellulose were modified with phosphotyrosine, a substrate for the brush border enzyme alkaline phosphatase. With these synthesized derivatives, different nanoparticle formulations, including the cystic fibrosis transmembrane conductance regulator gene were prepared by ionic gelation. Results: A change from negative to positive ζ potential after enzymatic cleavage could be observed. Transfection studies with HEK-293 and Caco-2 cells showed transfection rates comparable to Lipofectamine 2000. Transfection efficiencies were significantly decreased when phosphate cleavage and thus ζ potential change was inhibited by phosphatase inhibitor. Conclusion: The developed nanoparticles represent a promising gene delivery system.

Publication metadata

Author(s): Prufert F, Bonengel S, Kollner S, Griesser J, Wilcox MD, Chater PI, Pearson JP, Bernkop-Schnurch A

Publication type: Article

Publication status: Published

Journal: Nanomedicine

Year: 2017

Volume: 12

Issue: 22

Pages: 2713-2724

Print publication date: 01/11/2017

Online publication date: 29/09/2017

Acceptance date: 02/04/2016

Date deposited: 08/01/2018

ISSN (print): 1743-5889

ISSN (electronic): 1748-6963

Publisher: Future Medicine Ltd

URL: https://doi.org/10.2217/nnm-2017-0115

DOI: 10.2217/nnm-2017-0115

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