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Lookup NU author(s): Dr Matt WilcoxORCiD, Dr Peter Chater, Professor Jeffrey PearsonORCiD
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Future Medicine Ltd, 2017.
For re-use rights please refer to the publisher's terms and conditions.
© 2017 Future Medicine Ltd. Aim: Aim of the study was the development of ζ potential changing nanoparticles as gene delivery system for the cystic fibrosis transmembrane conductance regulator gene. Methods: Chitosan and carboxymethyl cellulose were modified with phosphotyrosine, a substrate for the brush border enzyme alkaline phosphatase. With these synthesized derivatives, different nanoparticle formulations, including the cystic fibrosis transmembrane conductance regulator gene were prepared by ionic gelation. Results: A change from negative to positive ζ potential after enzymatic cleavage could be observed. Transfection studies with HEK-293 and Caco-2 cells showed transfection rates comparable to Lipofectamine 2000. Transfection efficiencies were significantly decreased when phosphate cleavage and thus ζ potential change was inhibited by phosphatase inhibitor. Conclusion: The developed nanoparticles represent a promising gene delivery system.
Author(s): Prufert F, Bonengel S, Kollner S, Griesser J, Wilcox MD, Chater PI, Pearson JP, Bernkop-Schnurch A
Publication type: Article
Publication status: Published
Journal: Nanomedicine
Year: 2017
Volume: 12
Issue: 22
Pages: 2713-2724
Print publication date: 01/11/2017
Online publication date: 29/09/2017
Acceptance date: 02/04/2016
Date deposited: 08/01/2018
ISSN (print): 1743-5889
ISSN (electronic): 1748-6963
Publisher: Future Medicine Ltd
URL: https://doi.org/10.2217/nnm-2017-0115
DOI: 10.2217/nnm-2017-0115
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