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Lookup NU author(s): Professor William WillatsORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2017 The Author(s). Auxin is a key plant regulatory molecule, which acts upon a plethora of cellular processes, including those related to cell differentiation and elongation. Despite the stunning progress in all disciplines of auxin research, the mechanisms of auxin-mediated rapid promotion of cell expansion and underlying rearrangement of cell wall components are poorly understood. This is partly due to the limitations of current methodologies for probing auxin. Here we describe a click chemistry-based approach, using an azido derivative of indole-3-propionic acid. This compound is as an active auxin analogue, which can be tagged in situ. Using this new tool, we demonstrate the existence of putative auxin binding sites in the cell walls of expanding/elongating cells. These binding sites are of protein nature but are distinct from those provided by the extensively studied AUXIN BINDING PROTEIN 1 (ABP1). Using immunohistochemistry, we have shown the apoplastic presence of endogenous auxin epitopes recognised by an anti-IAA antibody. Our results are intriguingly in line with previous observations suggesting some transcription-independent (non-genomic) activity of auxin in cell elongation.
Author(s): Mravec J, Kracun SK, Zemlyanskaya E, Rydahl MG, Guo X, Picmanova M, Sorensen KK, Ruzicka K, Willats WGT
Publication type: Article
Publication status: Published
Journal: Scientific Reports
Year: 2017
Volume: 7
Issue: 1
Online publication date: 22/11/2017
Acceptance date: 10/11/2017
Date deposited: 13/12/2017
ISSN (print): 2045-2322
Publisher: Nature Publishing Group
URL: https://doi.org/10.1038/s41598-017-16281-w
DOI: 10.1038/s41598-017-16281-w
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