Toggle Main Menu Toggle Search

Open Access padlockePrints

Developing a Continuous Bioprocessing Approach to Stromal Cell Manufacture

Lookup NU author(s): Martina Miotto, Dr Ricardo Martins GouveiaORCiD, Professor Francisco FigueiredoORCiD, Professor Che ConnonORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2017 American Chemical Society. To this day, the concept of continuous bioprocessing has been applied mostly to the manufacture of molecular biologics such as proteins, growth factors, and secondary metabolites with biopharmaceutical uses. The present work now sets to explore the potential application of continuous bioprocess methods to source large numbers of human adherent cells with potential therapeutic value. To this purpose, we developed a smart multifunctional surface coating capable of controlling the attachment, proliferation, and subsequent self-detachment of human corneal stromal cells. This system allowed the maintenance of cell cultures under steady-state growth conditions, where self-detaching cells were continuously replenished by the proliferation of those remaining attached. This facilitated a closed, continuous bioprocessing platform with recovery of approximately 1% of the total adherent cells per hour, a yield rate that was maintained for 1 month. Moreover, both attached and self-detached cells were shown to retain their original phenotype. Together, these results represent the proof-of-concept for a new high-throughput, high-standard, and low-cost biomanufacturing strategy with multiple potentials and important downstream applications.

Publication metadata

Author(s): Miotto M, Gouveia R, Abidin FZ, Figueiredo F, Connon CJ

Publication type: Article

Publication status: Published

Journal: ACS Applied Materials and Interfaces

Year: 2017

Volume: 9

Issue: 47

Pages: 41131-41142

Print publication date: 29/11/2017

Online publication date: 17/11/2017

Acceptance date: 31/10/2017

Date deposited: 08/01/2018

ISSN (print): 1944-8244

ISSN (electronic): 1944-8252

Publisher: American Chemical Society


DOI: 10.1021/acsami.7b09809


Altmetrics provided by Altmetric


Funder referenceFunder name
BB/N021576/1Biotechnology and Biological Sciences Research Council (BBSRC)