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Effect of temperature on microbial diversity and nitrogen removal performance of an anammox reactor treating anaerobically pretreated municipal wastewater

Lookup NU author(s): Professor Russell DavenportORCiD, Professor David WernerORCiD



This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


The effects of temperature reduction (from 35 °C to 20 °C) on nitrogen removal performance and microbial diversity of an anammox sequencing batch reactor were evaluated. The reactor was fed for 148 days with anaerobically pretreated municipal wastewater amended with nitrite. On average, removal efficiencies of ammonium and nitrite were high (96%) during the enrichment period and phases 1 (at 35 °C) and 2 (at 25 °C), and slightly decreased (to 90%) when the reactor was operated at 20 °C. Deep sequencing analysis revealed that microbial community structure changed with temperature decrease. Anammox bacteria (Ca. Brocadia and Ca. Anammoximicrobium) and denitrifiers (Burkholderiales, Myxococcales, Rhodocyclales, Xanthomonadales, and Pseudomonadales) were favoured when the temperature was lowered from 35 °C to 25 °C, while Anaerolineales and Clostridiales were negatively affected. The results support the feasibility of using the anammox process for mainstream nitrogen removal from anaerobically pretreated municipal wastewater at typical tropical temperatures.

Publication metadata

Author(s): de Almeida Fernandes L, Pereira AD, Leal CD, Davenport R, Werner D, Mota Filho CR, Bressani-Ribeiro T, Augusto de Lemos Chernicharo C, Calabria de Araújo J

Publication type: Article

Publication status: Published

Journal: Bioresource Technology

Year: 2018

Volume: 258

Pages: 208-219

Print publication date: 01/06/2018

Online publication date: 23/02/2018

Acceptance date: 16/02/2018

Date deposited: 03/05/2018

ISSN (print): 0960-8524

ISSN (electronic): 1873-2976

Publisher: Elsevier BV


DOI: 10.1016/j.biortech.2018.02.083


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