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Changes in the proteome of the problem weed blackgrass correlating with multiple herbicide resistance

Lookup NU author(s): Dr Catherine Tétard-Jones, Professor Robert Edwards

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

Herbicide resistance in grass weeds is now one of the greatest threats to sustainable cereal production in Northern Europe. Multiple herbicide resistance (MHR), a poorly understood multigenic and quantitative trait, is particularly problematic as it provides tolerance to most classes of chemistries currently used for post‐emergence weed control. Using a combination of transcriptomics and proteomics, the evolution of MHR in populations of the weed blackgrass (Alopecurus myosuroides) has been investigated. While over 4500 genes showed perturbation in their expression in MHR vs. herbicide sensitive (HS) plants, only a small group of proteins showed >2‐fold changes in abundance, with a mere 8 proteins consistently associated with this class of resistance. Of the 8, orthologs of three of these proteins are also known to be associated with multiple drug resistance (MDR) in humans, suggesting a cross‐phyla conservation in evolved tolerance to chemical agents. Proteomics revealed that MHR could be classified into three sub‐types based on the association with resistance to herbicides with differing modes of action (MoA), being either global, specific to diverse chemistries acting on one MoA, or herbicide specific. Furthermore, the proteome of MHR plants were distinct from that of HS plants exposed to a range of biotic (insect feeding, plant‐microbe interaction) and abiotic (N‐limitation, osmotic, heat, herbicide safening) challenges commonly encountered in the field. It was concluded that MHR in blackgrass is a uniquely evolving trait(s), associated with changes in the proteome that are distinct from responses to conventional plant stresses, but sharing common features with MDR in humans.

Publication metadata

Author(s): Tétard-Jones C, Sabbadin F, Moss S, Hull R, Neve P, Edwards R

Publication type: Article

Publication status: Published

Journal: The Plant Journal

Year: 2018

Volume: 94

Issue: 4

Pages: 709-720

Print publication date: 01/05/2018

Online publication date: 25/03/2018

Acceptance date: 21/02/2018

Date deposited: 18/04/2018

ISSN (print): 0960-7412

ISSN (electronic): 1365-313X

Publisher: Wiley-Blackwell

URL: https://doi.org/10.1111/tpj.13892

DOI: 10.1111/tpj.13892

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